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Like all fresh water environments, Koi and Goldfish ponds are living ecosystems. They contain plants, algae, invertebrates, protozoa, bacteria and molds or fungi. Many of the life forms in our ponds are dependent upon each other. This is known as symbiosis (living together). An extreme form of symbiosis is parasitism, where one life form lives at the expense of another. One of the more common forms of parasitism in our ponds is Saprolegnia or water fungus. For the average ponder, great zoological detail about Saprolegnia is not required to understand what it is, what it does and how to treat the pond and fish if it becomes a problem, therefore not much detail on the zoology of Saprolegnia is offered here: just good-
What It Is: Saprolegnia (or sap) is a freshwater fungus which simply means it lives in fresh water environments and needs water to grow and reproduce. Sap can also be found in brackish water and moist soil. It is often referred to as a “cold water” fungus as it flourishes in colder water, but it lives well in a wide range of water temperatures extending from 37 deg F to 91 deg F (3 to 31 deg C). While it has been described also as a “mold”, sap is a genus of fungus, with the difference being a “mold” is a mass of fungi.
Under the microscope, sap is composed of filaments that tend to have spherical ends. It is these spherical ends that house the zoospores, or the “seeds” of sap, that allow it to proliferate and spread. The filaments are called hyphae and give sap its cotton-
In the water, sap looks like fluffy cotton; however, out of the water it appears to be a matted mess of slime. Sap starts out either white or grey in color. The grey appearance also may indicate the presence of bacteria growing with sap’s structure. Over a short period of time, sap may turn brown or green as organic particles in the water (such as algae) adhere to the filaments. Note the picture on the right. This is a classic presentation of sap that has invaded a bacterial infection of the dorsal fin area of a Koi. The green color is actually embedded algae particles within the sap’s filaments.
By appearance, sap can be easily confused with Epistylus, a freshwater parasite that presents as a white cottony substance growing on the skin of the fish. Epistylus does not gather organic particles and will remain white. Confirmation of Epistylus versus sap should be done with a microscope.
The favorite food of sap is dead organic tissue. We can usually see evidence of sap on dead and dying fish, live and dead fish eggs and even food left in the water so long that it has begun to rot. Typically we see infertile Koi eggs being infected first with the fungus then it spreads to kill live, fertile eggs. Infected eggs have a typical fluffy cotton wool-
Primary and Secondary Invader: Typically we think of sap as a “secondary” invader. This means that something else has violated the integrity of the fish’s skin allowing bacteria to enter and provide direct tissue access for the sap hyphae to embed themselves. In treating sap, it is imperative to treat BOTH the sap and the underlying primary cause. This will be discussed in more detail in the treatment section below.
However, sap can also be a primary invader under the right conditions and this is where it gets the reputation as a “cold water” fungus. We all know that cold water conditions, including dramatic temperature changes, cause great stress on the fish and suppress the fish’s natural immune system. As stated above, sap flourishes in colder water by producing and releasing increased zoospore counts into the water. The combination of stress, suppressed immune systems, and increased spore counts give sap the ability to cause major problems in colder water. Sap infections are commonly the cause of “winter kill.”
The following is a list of conditions that support the spread of sap:
Obviously the underlying theme in the above list is “stress” and this is the one thing that we need to guard against first and foremost to keep our fish healthy. In healthy conditions, our fish have some natural protection against sap with the mucous layer being the most effective first line of defense. The mucous layer provides the ability to reject a sap attack by sloughing off a layer of mucous and sending the sap with it. The mucous coat also provides a natural fungicide at the cell-
Death by SAP: An increased morbidity of fish caused by sap can be traced to three major things:
We know that sap attacks fish weakened by any of the above and the suppressed nature of the immune system under cold water or high stress conditions leaves the fish mostly defenseless. However, what kills the fish in a sap attack is a condition known as hemodilution. By definition, hemodilution is “a decreased concentration (as after hemorrhage) of cells and solids in the blood resulting from gain of fluid from the tissues.”, this causes the blood to loose electrolytes (blood salts) and make it less than life-
Once it has (literally!) taken root, sap can spread rapidly over the surface tissue of the fish. While it is rare that sap will penetrate deep into tissue layers, even superficial damage to the fish’s initial tissue layers (and particularly the gills) can be deadly. Obviously, the more wide-
Protection and Treatment: Obviously, the best “cure” for sap is prevention. As mentioned, sap is present in every pond and has its place in the order of life in the pond’s ecology. It really only becomes a problem when something has gone wrong with the fish and/or the pond or a condition such as cold water suppresses the fish’s immune system. Some of the steps we can take to reduce sap’s effectiveness include:
But no matter how hard we try, and especially for those ponds located in areas subject to cold water situations and/or rapid water temperature changes, sap is always a possibility. Except as noted above, there are no reasonable preventive measures, including the use of anti-
Treating a sap-
Much has been said regarding the importance of using a microscope in the Koi hobby. A microscope with a range up to 400x (eg 40X objective and 10X eyepiece) is an invaluable tool for diagnosing problems with our fish as well as viewing almost the entire spectrum of the ecology of our ponds from algae to parasites to the larvae of insects. At 200x, the filament structure of sap is easily to see and at 400x, the hyphae and spherical ends of sap are clearly discernible. Also, at 200x the movement of most parasites can be easily seen and at 400x, virtually all of the parasites that routinely inhabit our ponds can be detected and identified.
As you can see, sap is one of the major potential problems that we have to be prepared to deal with. Since sap is always present in our ponds, it is imperative to take as many preventative measures as possible to keep it from affecting the fish. But even the best pond keepers will eventually get a sap outbreak and when this happens, treatment must be quick and complete as this is not a problem that will just go away all by itself.
Within this category of “Fish Health” here on koisale.com we wish to thank Dr. Roy Yanong from the University of Florida IFAS Extension for his permission to utilize much of the information contained here-
Knowing when to seek help or When and How to Look for Fish Veterinarian
This is the website for the American Association of Fish Veterinarians (which several friends and I formed a few years ago, and now has over 130 fish vet members around the U.S.). We are still inputting data for the find-
For more detailed information click here for the .pdf file from UF|IFAS